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KMID : 0811720020060000172
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Korean Journal of Physiology & Pharmacology
2002 Volume.6 No. 0 p.172 ~ p.0
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Expression of Dendroaspis Natriuretic Peptide System in Rat
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Kim Yoon-Ah
Kim Soo-Mi
Kim So-Young
Park Sung-Hun
Kim Sun-Hee
Cho Kyung-Woo
Kim Sung-Zoo
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Abstract
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Dendroaspis natriuretic peptide (DNP) is a recently discovered 38-amino acid residue peptide, isolated from the snake, with structural similarity to ANP, BNP, and CNP. Recently, immunoreactive DNP (ir-DNP) was detected in plasma, atrial myocardium, and vascular smooth muscle of mammals including human. However, the synthesis of DNP, its specific binding sites, and a physiological role are yet to be defined. Our objectives were to define the expression of ir-DNP and its specific receptors in various tissues of rat. The ir-DNP was detected in various tissues including cardiac atrium and ventricles, brain, liver, kidney, spleen, and intestine. Plasma concentration was about 200 pg/ml. These tissue contents and plasma concentrations of DNP were not different between male and female rats. Even though DNP contents in cardiac atrium (about 15 pg/mg wet weight) were higher than those in other tissues, we have not found major tissue for DNP synthesis and/or secretion. The molecular structure of DNP in blood was more stable rather than those of ANP. By quantitative in vitro receptor autoradiography, specific DNP binding sites were found in heart (right and left ventricular endocardium), kidney (glomeruli, inner medullar, interlobular artery, and vasa recta bundle), liver (hepatic artery), and brain (olfactory bulb, cingulate cortex, corpus callosum, anterior commissure, and arachoid mater). In renal glomerulus and inner medullar, and olfactory bulb, DNP could be produced cGMP via activation of particulate guanylyl cyclase. Affinity cross-linking of renal glomerular membranes with radiolabeled ANP followed by SDS-PAGE under reducing conditions showed that the receptor molecules for ANP migrated as two bands at a position of about 140 and 70 kDa. The addition of a excess concentration of unlabeled DNP totally displaced radiolabeled ANP from both bands. These results indicate that ir-DNP is expressed in various tissues, and support that DNP may be act as autocrine and/or paracrine hormone in heart, kidney, and brain of rat. (Supported by KRF Grant 2001-041-F00039)
Source: Korean Journal of Physiology & Pharmacology.2002 Oct;6(Suppl II):S115-S115
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KEYWORD
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DNP, receptor, autoradiography, cGMP, cross-linking
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